For detection of 25-hydroxyvitamin D- 25(OH)D which is the main storage form of vitamin D in the body. Therefore, the overall presence of vitamin D can be determined by detecting the content of 25(OH)D. 25(OH)D levels less than 30 ng/mL may indicate Vitamin D Deficiency or Insufficiency. Vitamin D supplements may be recommended in these cases of vitamin D deficiency and monitoring of effectiveness of supplements can be tested for with this test.
The Vitamin D Rapid Test Cassette (Fingerstick Whole Blood) test mixture migrates upwards on the strip by capillary action. The membrane is precoated with 25(OH)D antigens on the test line region of the strip. During testing, 25(OH)D present in the specimen will compete with 25(OH)D on the test line. The higher concentration of 25(OH)D in the specimen, the lighter would be the T line. The result will be read according to the colour card provided with the kit. To serve as a procedural control, a coloured line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
